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Method for measuring a comprehensive energy budget in a proliferating cell system over multiple cell cycles
Author(s) -
Guppy Michael,
Kong SungEun,
Niu Xianwa,
Busfield Samantha,
Klinken S. Peter
Publication year - 1997
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199701)170:1<1::aid-jcp1>3.0.co;2-s
Subject(s) - cell , energy budget , energy metabolism , range (aeronautics) , production (economics) , metabolite , biological system , energy consumption , measure (data warehouse) , computer science , environmental science , process engineering , chemistry , microbiology and biotechnology , biochemistry , biology , materials science , data mining , engineering , ecology , macroeconomics , economics , composite material , endocrinology
Isolated cell systems are now being used very effectively to study a range of important biochemical questions, but their energy metabolism has never been comprehensively investigated. We have developed a system, using J2E cells, which enables us to measure total ATP turnover and the contribution of various fuels and pathways to this total in a dynamic, proliferating preparation. Cells are cultured in 500 ml airtight glass containers which enables (1) the measurement of oxygen consumption, (2) the collection and measurement of 14 CO 2 production from labelled fuels, and (3) the measurement of metabolite utilization and production. Data on cell numbers are then used to produce a curve of cell number vs. time, the area under which (cell numbers · hour) is used as a base by which all measurements and experiments are compared. To our knowledge this is the first time a comprehensive energy budget has been measured in a proliferating cell system over a period that covers multiple cell cycles. J Cell Physiol 170:1–7, 1997 © 1997 Wiley‐Liss, Inc.