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Phospholipase C β2 in vascular smooth muscle
Author(s) -
Labelle Edward F.,
Polyák Erzsébet
Publication year - 1996
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199611)169:2<358::aid-jcp15>3.0.co;2-5
Subject(s) - phospholipase c , phospholipase , vascular smooth muscle , biology , endocrinology , gene isoform , medicine , phospholipase a2 , pld2 , inositol , receptor , biochemistry , phosphatidylcholine , enzyme , phospholipid , membrane , smooth muscle , gene
Receptor‐mediated inositol 1,4,5‐trisphosphate formation in most tissues is dependent on a variety of phospholipase C isoforms. To determine which phospholipase C isoforms were present in vascular smooth muscle compared to brain, liver, and spleen, we extracted proteins from these tissues and separated and identified the phospholipase C isoforms by immunoblotting. Aliquots of rat tail artery were examined by this procedure, together with aliquots of rat liver, spleen, cerebral cortex, hippocampus, cerebellum, aorta, and mesenteric artery. Phospholipase C γ1 was shown to be present in all of these tissues, while phospholipase C β1 was shown to be limited to fractions from brain. Phospholipase C Δ1 was detected in rat tail artery, mesenteric artery, aorta, and brain. Phospholipase C β2 was found in rat tail artery, liver, and brain. This is the first report of phospholipase C β2 in tissues other than HL60 cells. Since G proteins activate IP 3 production via stimulation of phospholipase Cβ isoforms in many tissues, and agonist‐stimulated IP 3 production in smooth muscle requires G protein activation, phospholipase C β2 may be required for agonist‐stimulated force production in vascular smooth muscle. © 1996 Wiley‐Liss, Inc.