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Synergistic effects of substance P with insulin‐like growth factor‐1 on epithelial migration of the cornea
Author(s) -
Nishida Teruo,
Nakamura Masatsugu,
Ofuji Keiko,
Reid Ted W.,
Mannis Mark J.,
Murphy Christopher J.
Publication year - 1996
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199610)169:1<159::aid-jcp16>3.0.co;2-8
Subject(s) - substance p , endocrinology , medicine , receptor , corneal epithelium , fibronectin , biology , cornea , epithelium , microbiology and biotechnology , growth factor , extracellular matrix , chemistry , neuropeptide , genetics , neuroscience
We find that substance P (SP) and insulin‐like growth factor‐1 (IGF‐1) demonstrate a synergistic effect on the stimulation of rabbit corneal epithelial migration in an organ culture. The addition of either SP or IGF‐1 alone did not affect epithelial migration, while the combination of SP and IGF‐1 stimulated epithelial migration in a dose‐dependent fashion. The synergistic effects of SP and IGF‐1 on corneal epithelial migration were nulled by the addition of a SP antagonist or enkephalinase. Among neurotransmitters (vasoactive intestinal peptide, calcitonin gene‐related peptide, acethylcholine chloride, norepinephrine, serotonin) or tachykinins (neurokinin A, neurokinin B, kassinin, eledoisin, physalaemin), only SP demonstrated a synergistic effect with IGF‐1 on cellular migration. In contrast, the combination of SP and IGF‐1 did not affect the incorporation of 3 H‐thymidine into corneal epithelial cells. The attachment of the corneal epithelial cells to fibronectin, collagen type IV, and laminin matrices increased after treatment of the cells with SP and IGF‐1, but SP or IGF‐1 by themselves did not affect the attachment of the cells to these extracellular matrix proteins. An identical synergistic effect on corneal epithelial migration was observed when an NK‐1 receptor agonist was used in place of SP, suggesting the synergistic effect of SP and IGF‐1 might be mediated through the NK‐1 receptor system. These results suggest that the maintenance of the normal integrity of the corneal epithelium might be regulated by both humoral and neural factors. © 1996 Wiley‐Liss, Inc.