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Enhanced biosynthesis of extracellular matrix proteins and TGF‐β1 by polyinosinic‐polycytidylic acid during cutaneous wound healing in vivo
Author(s) -
Sidhu Gurmel S.,
Thaloor Deepa,
Singh Anoop K.,
Raghunath Puthiyaveettil N.,
Maheshwari Radha K.
Publication year - 1996
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199610)169:1<108::aid-jcp11>3.0.co;2-9
Subject(s) - wound healing , extracellular matrix , laminin , cell adhesion molecule , chemistry , cell adhesion , fibronectin , microbiology and biotechnology , adhesion , biochemistry , immunology , cell , biology , organic chemistry
In our previous study, we have shown that polyinosinic‐polycytidylic acid (poly I:C), a double‐stranded RNA, and a potent inducer of interferon, enhanced the wound healing in rats and mice. Increased levels of laminin and collagen, and greater influx of dermal fibroblasts were observed in poly I:C‐treated wounds as compared to untreated wounds (Bhartiya et al., 1992, J. Cell. Physiol., 150 :312–319). In this study, we have explored the mechanism of enhanced wound healing by poly I:C in rats. Poly I:C (1 mg/kg) in phosphate buffered saline was injected intraperitoneally 18 h prior to wound healing, and the animals were sacrificed on day 3 postwounding. Immunofluorescence studies showed increased expression of adhesion molecules that includes ICAM‐1 (intercellular adhesion molecule‐1; CD54) and VCAM‐1 (vascular cell adhesion molecule; CD 106) in poly I:C‐treated wounds as compared to untreated control. Poly I:C treatment resulted in an increase in the mRNA levels of collagen type 1 (α), collagen III, laminin B1, and transforming growth factor‐beta 1 (TGF‐β1) in wounds compared to untreated wounds as demonstrated by in situ hybridization and PCR analysis. These studies suggests that poly I:C upregulates the biosynthesis of adhesion molecules, extracellular matrix proteins (ECM), and TGF‐β1 in the wound bed. Adhesion molecules and ECM play a major role in wound healing, and TGF‐β1 has been known to be a potent wound healer. Therefore, the increased expression of these molecules may play a role in the enhanced healing by poly I:C observed in rats. © 1996 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.