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Supplemental L‐arginine HCI augments bacterial phagocytosis in human polymorphonuclear leukocytes
Author(s) -
Moffat Frederick L.,
Han Tieran,
Li ZhiMing,
Peck Michael D.,
Jy Wenche,
Ahn Yeon S.,
Chu Arthur J.,
Bourguig Lilly Y. W.
Publication year - 1996
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199607)168:1<26::aid-jcp4>3.0.co;2-a
Subject(s) - phagocytosis , arginine , immune system , nitric oxide , phagocyte , granulocyte , biology , chemistry , biochemistry , microbiology and biotechnology , immunology , amino acid , endocrinology
That L‐arginine (L‐Arg) augments the host response to acute bacterial sepsis suggests that this amino acid intervenes early in the immune response, perhaps via the nitric oxide synthetase (NOS) pathway. The effect of L‐Arg supplementation on in vitro phagocytosis of fluorescein‐labeled, heat‐killed Staphylococcus aureus by peripheral blood neutrophils (PMNs) from 12 normal human volunteers was studied. Separated PMNs were incubated for 2 h with labeled bacteria, with and without supplemental L‐Arg, D‐arginine, glycine, and/or the NOS inhibitors L‐canavanine, aminoguanidine, or L‐N G ‐nitroarginine methyl ester. PMNs were fixed and extracellular fluorescence quenched with crystal violet. By flow cytometry and confocal microscopy, L‐Arg supplementation was shown to result in a highly significant increase in PMN bacterial phagocytosis, the maximal effect being seen with L‐Arg 380 μM and falling off with higher concentrations. This augmentation was completely abrogated by NOS inhibitors in molar excess, but inhibitors alone did not suppress phagocytosis below that of unsupplemented controls. Neither D‐arginine nor glycine affected phagocytosis; the L‐Arg effect was stereospecific and not related to utilization of L‐Arg as an energy source. L‐Arg supplementation significantly enhances bacterial phagocytosis in human neutrophils, perhaps by effects on cytoskeletal phenomena, and this appears to be mediated through NOS activity. Phagocytosis by nonspecific immune cells which intervene early in the response to sepsis is critically important, and beneficial effects of L‐Arg on the clinical course of sepsis may be due at least in part to augmentation of phagocyte function. © 1996 Wiley‐Liss, Inc.