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Deletion of specific protein kinase C subspecies in human melanoma cells
Author(s) -
Oka Masahiro,
Ogita Kouji,
Ando Hideya,
Horikawa Tatsuya,
Hayashibe Kazuhito,
Saito Naoaki,
Kikkawa Ushio,
Ichihashi Masamitsu
Publication year - 1996
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199606)167:3<406::aid-jcp4>3.0.co;2-t
Subject(s) - protein kinase c , pkc alpha , melanoma , cell culture , cell growth , biology , tetradecanoylphorbol acetate , microbiology and biotechnology , beta (programming language) , alpha (finance) , cancer research , melanocyte , signal transduction , medicine , biochemistry , genetics , construct validity , nursing , computer science , patient satisfaction , programming language
It has been shown that tumor‐promoting phorbol ester, 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), stimulates the proliferation of normal human melanocytes, whereas it inhibits the growth of human melanoma cell lines. The expression of protein kinase C (PKC) subspecies, the major intracellular receptors for TPA, was examined in normal melanocytes and the four melanoma cell lines HM3KO, MeWo, HMV‐1, and G361. PKC was partially purified and then separated into subspecies by column chromatography on Mono Q and hydroxyapatite successively, and finally subjected to immunoblot analysis using antibodies specific for the PKC subspecies. Of the PKC subspecies examined, δ‐, ϵ‐, and ζ‐PKC were detected in both normal melanocytes and the four melanoma cell lines. In contrast, both α‐PKC and β‐PKC were expressed in normal melanocytes, whereas either α‐PKC or β‐PKC was detected in melanoma cells. Specifically, HM3KO, MeWo, and HMV‐1 cells were shown to contain α‐PKC but not β‐PKC, while G361 cells expressed β‐PKC but not α‐PKC. The growth of these melanoma cells was suppressed by TPA treatment, and the growth of the G361 cells lacking α‐PKC was inhibited more efficiently than the other melanoma cell lines which lacked β‐PKC. It was further shown that β‐PKC was not detected in freshly isolated human primary or metastatic melanoma tissues. These results suggest that the expression of α‐PKC or β‐PKC may be altered during the malignant transformation of normal melanocytes and that loss of α‐PKC or β‐PKC may be related to the inhibitory effect of TPA on the growth of melanoma cells. © 1996 Wiley‐Liss, Inc.