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Suppression of UV‐ and interferon‐α‐refractoriness by antipain in human IF r cells established from RSa cells sensitive to both stimuli
Author(s) -
Suzuki Nobuo
Publication year - 1996
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199604)167:1<47::aid-jcp5>3.0.co;2-c
Subject(s) - antipain , cell culture , protease , in vitro , proteases , biology , cell , irradiation , microbiology and biotechnology , chemistry , enzyme , biochemistry , leupeptin , genetics , physics , nuclear physics
The human cell line IF r is a variant with an increased resistance to cell proliferation inhibition (CPI) by human interferon (HuIFN)‐α, established from RSa cells with unusually high‐sensitivity to CPI. IF r cells were later found to have increased resistance to the cell‐killing effects of far‐ultraviolet (UV) irradiation. Here, in cell lysates extracted from UV‐irradiated IF r cells but not in those from irradiated RSa cells, fibrinolytic protease activity was found to be elevated promptly and transiently after irradiation. Treatment of IF r cells with HuIFN‐α alone also resulted in the elevation of protease activity, but not that of RSa cells. Both the activity elevated after UV irradiation and after HuIFN‐α treatment was inhibited to the greatest extent by antipain in vitro. Moreover, the refractoriness of IF r cells to UV cell‐killing and to HuIFN‐α CPI was suppressed by culturing with medium containing antipain immediately after UV irradiation or during HuIFN‐α exposure. In similarly treated RSa cells, there was no modulation of UV‐ or HuIFN‐α‐susceptibility. These comparative characteristics between the two cell lines suggested that antipain‐sensitive proteases and/or cellular functions may be involved in increased resistance to UV and HuIFN‐α of IF r cells. © 1996 Wiley‐Liss, Inc.