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Opposite regulation of thrombospondin‐1 and corticotropin‐induced secreted protein/thrombospondin‐2 expression by adrenocorticotropic hormone in adrenocortical cells
Author(s) -
Lafeuillade Bruno,
Pellerin Sylvie,
Keramidas Michelle,
Danik Marc,
Chambaz Edmond M.,
Feige JeanJacques
Publication year - 1996
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199604)167:1<164::aid-jcp19>3.0.co;2-b
Subject(s) - adrenocorticotropic hormone , messenger rna , biology , medicine , endocrinology , complementary dna , adrenal cortex , thrombospondin , immunoprecipitation , microbiology and biotechnology , antibody , hormone , gene , biochemistry , immunology , metalloproteinase , matrix metalloproteinase
Corticotropin‐induced secreted protein (CISP) is a trimeric glycoprotein secreted by primary cultures of bovine adrenocortical cells in response to adrenocorticotropic hormone (ACTH). This protein was recently purified in our laboratory, and its N‐terminal amino‐acid sequence revealed a significant similarity with thrombospondin‐2 (TSP2). We report here the nucleotide sequence of a 386 bp RT‐PCR fragment specific for CISP. The deduced protein sequence shares 84% identity with the N‐terminal portion of mature human TSP2, suggesting that CISP is its bovine counterpart. Northern analysis of adrenocortical cell RNA using the above cDNA fragment as a probe revealed a 6.0 kb CISP/TSP2 mRNA whose abundance was increased nearly fivefold following a 24 h cell treatment with 10 −7 M ACTH. Under the same conditions, the expression of TSP1 mRNA was reduced by ten‐fold. The protein levels of TSP1 and CISP/TSP2 varied accordingly with their respective mRNA levels, as shown by immunoprecipitation and immunofluorescence experiments. Taken together, these data show that ACTH induces a dramatic shift in the pattern of adrenocortical cell thrombospondin expression from TSP1 to CISP/TSP2. This observation suggests that these two members of the thrombospondin family exert distinct biological functions in the adrenal cortex. This hypothesis is further supported by the observation that anti‐CISP antibodies inhibit the maintenance of the morphological changes of bovine adrenocortical cells induced by ACTH, whereas anti‐TSP1 antibodies do not. © 1996 Wiley‐Liss, Inc.