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Localized calcium signaling in multinucleated osteoclasts
Author(s) -
Xia ShenLing,
Ferrier Jack
Publication year - 1996
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199604)167:1<148::aid-jcp17>3.0.co;2-7
Subject(s) - cytoplasm , multinucleate , cytosol , intracellular , osteoclast , calcium , biophysics , microbiology and biotechnology , cell , calcium in biology , chemistry , biology , biochemistry , organic chemistry , in vitro , enzyme
Localized intracellular Ca 2+ ([Ca 2+ ] i ) pulses, fluctuations, and repetitive spikes were detected in multinucleated rabbit osteoclasts in the presence of serum and in response to calcitonin using the fluorescent calcium indicator fluo‐3 and a laser scanning microscope. We observed that these [Ca 2+ ] i changes were often restricted within a region of the cell body or propagated from the initial region of occurrence to other parts of the cell body but not to all parts. These observations suggest the existence of significant barriers to Ca 2+ transport between different cytoplasmic regions of the osteoclast. To further investigate this phenomenon, we mechanically perturbed different cellular regions by touching locally with a micropipette. This usually induced a local increase in cytosolic and nuclear free [Ca 2+ ] i . In some cases there was propagation of the [Ca 2+ ] i increase to other regions but with part of the cell body not affected. Those regions of the cell body to which the [Ca 2+ ] i increase did not propagate had a [Ca 2+ ] i response to a direct mechanical perturbation. Our data show that osteoclasts can have different [Ca 2+ ] i activities in apparently equivalent cellular regions, no matter how generated. This suggests that there can be a number of spatially separate Ca 2+ regulatory systems within an osteoclast cell body. © 1996 Wiley‐Liss, Inc.