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Modulation of α5β1 integrin functions by the phospholipid and cholesterol contents of cell membranes
Author(s) -
Gopalakrishna P.,
Chaubey S.K.,
Manogaran P.S.,
Pande Gopal
Publication year - 2000
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(20000615)77:4<517::aid-jcb1>3.0.co;2-6
Subject(s) - fibronectin , microbiology and biotechnology , integrin , focal adhesion , stress fiber , chemistry , cytoskeleton , cell adhesion , biochemistry , biology , cell , extracellular matrix , signal transduction
Several modifications of the α5β1 integrin, which alter its intracellular and extracellular interaction with fibronectin and other proteins, have been reported. However, the significance of the lateral mobility of integrin molecules in the plasma membrane, as a regulator of their distribution and function, is poorly understood. We examined this problem by increasing the cholesterol content of plasma membranes, and consequently modifying the fluidity of membrane phospholipids, in rat fibroblasts. Under these conditions, the clustering of α5β1 integrin molecules in focal adhesions, their adhesion to the cell‐binding domain of fibronectin, and their association with the cytoskeletal protein talin were significantly enhanced as compared to control cells. However, the activation of MAP‐kinase pathways by the association of fibronectin with α5β1 integrin, and its association with integrin‐linked kinase (ilk), were suppressed. The treated cells also showed distinct changes in shape, and their actin stress fiber network was more dense and thick as compared to control cells. The changes in fluidity of phospholipids occurred differentially and fluidity of phosphatidyl‐ethanolamine increased, while that of phosphatidyl‐choline was reduced. Our results suggest that proteins in focal adhesions could be partitioned in specific lipid domains, which regulate specific aspects of α5β1 integrin functions. J. Cell. Biochem.77:517–528, 2000. © 2000 Wiley‐Liss, Inc.