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Defective growth in vitro of Duchenne muscular dystrophy myoblasts: The molecular and biochemical basis
Author(s) -
Melone Mariarosa A.B.,
Peluso Gianfranco,
Petillo Orsolina,
Cotrufo Roberto
Publication year - 2000
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(20000101)76:1<118::aid-jcb12>3.0.co;2-f
Subject(s) - duchenne muscular dystrophy , myocyte , muscular dystrophy , microbiology and biotechnology , regeneration (biology) , transforming growth factor , pathogenesis , biology , autocrine signalling , in vitro , dystrophin , cancer research , chemistry , cell culture , immunology , genetics
As the molecular basis of Duchenne Muscular Dystrophy (DMD) was being discovered, increasing focus was placed on the mechanisms of progressive failure of myoregeneration. In this study, we propose a pathogenesis model for DMD, where an autocrine growth factor release of TGF‐β1—from necrotic myofibers—could contribute to the increasing loss of muscle regeneration. In fact, we report evidence that DMD myoblasts reduce their proliferation rate, in time and later cultures; in connection with this, we observed TGF‐β1 increase in conditioned media of DMD myoblasts, able to control the myoblast growth by reducing fusion and differentiation of DMD satellite cells. J. Cell. Biochem. 76:118–132, 1999. © 1999 Wiley‐Liss, Inc.