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New role for tRNA and its fragment purified from human urinary bladder carcinoma conditioned medium: Inhibition of endothelial cell growth
Author(s) -
Zhao Hui,
Bojanowski Krzysztof,
Ingber Donald E.,
Panigrahy Dipak,
Pepper Michael S.,
Montesano Roberto,
Shing Yuen
Publication year - 2000
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(20000101)76:1<109::aid-jcb11>3.0.co;2-k
Subject(s) - in vitro , rna , endothelial stem cell , transfer rna , cell culture , cell growth , angiogenesis , biology , microbiology and biotechnology , cell , ribosomal rna , protein biosynthesis , chemistry , biochemistry , cancer research , gene , genetics
The growth of endothelial cells is necessary for angiogenesis, which in turn is required for later steps of tumor progression. In an attempt to purify new modulators of endothelial cell growth from the conditioned medium of human urinary bladder carcinoma cells, we isolated a small and stable oligonucleotide containing 10 to 16 bases. This oligonucleotide inhibited the growth of endothelial cells in vitro and was identified as a fragment of transfer RNA (tRNA). When unfractionated bovine tRNA was added to the cell culture, it specifically inhibited growth of endothelial cells, but not smooth muscle cells, bovine kidney cells, 3T3 fibroblasts, and several cancer cell lines. In contrast, ribosomal RNA, total yeast RNA, and single nucleosides from tRNA hydrolysate had no effect. These results demonstrate a new role for tRNA and its fragment as a selective endothelial cell inhibitor in vitro. J. Cell. Biochem. 76:109–117, 1999. © 1999 Wiley‐Liss, Inc.