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Endpoint markers for cancer chemoprevention trials derived from the lesion of precancer (intraepithelial neoplasia) measured by computer‐assisted quantitative image analysis
Author(s) -
Boone Charles W.,
Kelloff Gary J.
Publication year - 2000
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(2000)77:34+<67::aid-jcb12>3.0.co;2-j
Subject(s) - magnification , lesion , cancer , biology , nuclear dna , pathology , nuclear medicine , medicine , physics , genetics , optics , gene , mitochondrial dna
Endpoint markers for cancer chemoprevention clinical trials are described that are developed from the morphological properties of the precancerous lesion of intraepithelial neoplasia itself, as measured by computer‐assisted quantitative image analysis. The markers include increased proliferative fraction (percentage MIB‐1 positive nuclear area); nuclear DNA content (DNA ploidy); including DNA content exceeding fivefold the haploid amount (5C‐exceeding rate); nuclear/nucleolar morphometry; and disorganization of nuclear chromatin pattern as characterized by Markovian parameters and other functions. A significant new advance in image analysis is the process of "tiling", in which hundreds of full monitor image fields of a given histological section at ×40 magnification are reduced in size and fused seamlessly to produce a single image of the histological section at ×1.25 magnification. The operator may review the low‐power image and retrieve ×40 magnification of any desired area by point/clicking with a mouse. J. Cell. Biochem. Suppl. 34:67–72, 2000. Published 2000 Wiley‐Liss, Inc.