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Effects of electrical fields on cardiomyocyte differentiation of embryonic stem cells
Author(s) -
Sauer Heinrich,
Rahimi Gohar,
Hescheler Jürgen,
Wartenberg Maria
Publication year - 1999
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19991215)75:4<710::aid-jcb16>3.0.co;2-z
Subject(s) - embryoid body , embryonic stem cell , microbiology and biotechnology , chemistry , intracellular , depolarization , biophysics , biology , induced pluripotent stem cell , biochemistry , gene
The effects of electromagnetic fields (EMFs) on the differentiation of cardiomyocytes in embryoid bodies derived from pluripotent embryonic stem (ES) cells were investigated. A single direct current (DC) field pulse was applied to 4‐day‐old embryoid bodies. The electrical field induced a hyperpolarization of the anode‐facing side of embryoid bodies and a depolarization at the cathode‐facing side. Significant effects of a single electrical field pulse applied for 90 s on cardiomyocyte differentiation were achieved with field strengths of 250 and 500 V/m, which increased both the number of embryoid bodies differentiating beating foci of cardiomyocytes and the size of the beating foci. The 500‐V/m electrical field increased intracellular reactive oxygen species (ROS), but not [Ca 2+ ] i and activated nuclear factor kappa B (NF‐κB). A comparable increase in the number of beating embryoid bodies was achieved by an incubation for 1 h with H 2 O 2 (1–10 nM), indicating that the electrical field effect was transduced via the intracellular generation of ROS. Because the radical scavengers dehydroascorbate and pyrrolidinedithiocarbamate (APDC) and the NF‐κB antagonist N‐tosyl‐ L ‐phenylalanine chloromethyl ketone (TPCK) inhibited cardiac differentiation, we assume that ROS and NF‐κB may play a role in early cardiac development. J. Cell. Biochem. 75:710–723, 1999. © 1999 Wiley‐Liss, Inc.