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Abnormal osteogenesis in osteoporotic patients is reflected by altered mesenchymal stem cells dynamics
Author(s) -
Rodríguez J. Pablo,
Garat Solange,
Gajardo Héctor,
Pino Ana María,
Seitz Germán
Publication year - 1999
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19991201)75:3<414::aid-jcb7>3.0.co;2-c
Subject(s) - mesenchymal stem cell , stem cell , alkaline phosphatase , bone marrow , stem cell transplantation for articular cartilage repair , clinical uses of mesenchymal stem cells , immunology , microbiology and biotechnology , medicine , biology , adult stem cell , cancer research , in vitro , endothelial stem cell , biochemistry , enzyme
Bone marrow contains a population of mesenchymal stem cells with the ability to differentiate into cells that form bone, cartilage, adipose, and other connective tissues. Stem cells can be isolated from bone marrow aspirates and expanded in vitro. Presently, most stem cells studies have been performed in cells obtained from “healthy” control subjects. The goal of this study was to compare the functional characteristics of mesenchymal stem cells derived from “healthy” control and osteoporotic postmenopausal women to better understand the mechanisms involved in the pathogenesis of this disease. Osteoporotic and control stem cells have similar morphology and size and express similar cell surface antigens as evidenced by their reactivity with cell specific monoclonal antibodies. Mesenchymal stem cells from osteoporotic women differ from controls in having a lower growth rate than control cells, being refractory to the mitogenic effect of IGF‐1, and exhibiting a deficient ability to differentiate into the osteogenic linage as evidenced by the alkaline phosphatase activity and calcium phosphate deposition. We conclude that in osteoporosis stem cell growth, proliferative response and osteogenic differentiation are significantly affected. Also, the study of mesenchymal stem cells from osteoporotic postmenopausal women may provide a better understanding of the mechanisms involved in the pathogenesis of the osteoporosis. It may also serve to test in vitro in rapid manner novel new therapeutic strategies. J. Cell. Biochem. 75:414–423, 1999. © 1999 Wiley‐Liss, Inc.