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Modulation of apo A‐IV transcript levels and synthesis by n‐3, n‐6, and n‐9 fatty acids in CACO‐2 cells
Author(s) -
Stan Simona,
Delvin Edgard E.,
Seidman Ernest,
Rouleau Thérèse,
Steinmetz Armin,
Bendayan Moïse,
Yotov Wagner,
Levy Emile
Publication year - 1999
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19991001)75:1<73::aid-jcb8>3.0.co;2-k
Subject(s) - modulation (music) , caco 2 , chemistry , microbiology and biotechnology , biochemistry , biology , cell , physics , acoustics
It has been postulated that apolipoprotein (apo) A‐IV plays various significant roles in lipid transport and lipoprotein metabolism. Although it is controlled by fat feeding, so far little else is known about its regulation by specific fatty acids. In this study, we focused on the modulation of apo A‐IV mRNA levels, mass, and biogenesis by mono‐ and polyunsaturated fatty acids (FA) in the human intestinal Caco‐2 cell line. In confluent cells incubated with 1 mM oleic (n‐9), linoleic (n‐6), α‐linolenic (n‐3), or docosahexaenoic (n‐3) acids for a long‐term period, both apo A‐IV protein levels and de novo synthesis were increased. The induction resulted from the up‐regulation of apo A‐IV mRNA transcripts. In contrast, an inhibitory effect was evident with short‐term incubation. FA chain length and degree of unsaturation had little effect altering apo A‐IV transcript and biogenesis. These data offer evidence that isolated fatty acids regulate gene expression and the production of apo A‐IV in the enterocyte. J. Cell. Biochem. 75:73–81, 1999. © 1999 Wiley‐Liss, Inc.