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Simple preparation method of PCR fragments for automated DNA sequencing
Author(s) -
Høgdall Estrid,
Boye Kit,
Vuust Jens
Publication year - 1999
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19990615)73:4<433::aid-jcb1>3.0.co;2-q
Subject(s) - primer (cosmetics) , polymerase chain reaction , digital polymerase chain reaction , dilution , primer dimer , precipitation , chromatography , dna , ethanol precipitation , dna sequencing , chemistry , in silico pcr , polymerase chain reaction optimization , microbiology and biotechnology , biology , computational biology , nested polymerase chain reaction , biochemistry , gene , multiplex polymerase chain reaction , physics , meteorology , extraction (chemistry) , organic chemistry , thermodynamics
In an effort to find a simple and inexpensive purification method of polymerase chain reaction (PCR) reaction before cycle sequencing reaction, we compared a commercial system with a precipitation protocol performed in our laboratory. We found that, particularly with small PCR products, our method works with greater success than the method compared. Our precipitation method may be used on a larger PCR fragment before cycle sequencing reaction as well. Furthermore, it has the advantage of being simple as the well‐known dilution method; in contrast to the dilution method, the precipitation method removes excess primers as well as possible primer dimers. J. Cell. Biochem. 73:433–436, 1999. © 1999 Wiley‐Liss, Inc.