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Palmitoyl ascorbate: Selective augmentation of procollagen mRNA expression compared with L ‐ascorbate in human intestinal smooth muscle cells
Author(s) -
Rosenblat Gennady,
Willey Amy,
Zhu YaNan,
Jonas Adi,
Diegelmann Robert F.,
Neeman Ishak,
Graham Martin F.
Publication year - 1999
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19990601)73:3<312::aid-jcb3>3.0.co;2-1
Subject(s) - procollagen peptidase , ascorbic acid , chemistry , biochemistry , sodium ascorbate , messenger rna , in vitro , microbiology and biotechnology , biology , gene , food science
The effect of 6‐O‐palmitoyl ascorbate on procollagen mRNA levels, collagen synthesis, and collagen secretion was investigated and compared with the effect of l ‐ascorbate in human intestinal smooth muscle (HISM) cells in vitro. Collagen synthesis, determined by the incorporation of 3 H‐proline into pepsin‐resistant, salt‐precipitated collagen, increased in a concentration‐dependent manner in response to palmitoyl ascorbate. There was a twofold increase in collagen synthesis at 2.5 and 5 μM. By contrast, l ‐ascorbate was required at 4–5 times the concentration for the same response. However, at 20 μM, both palmitoyl and l ‐ascorbate induced similar 2.7‐fold increases in collagen synthesis. Palmitoyl ascorbate induced a 1.6‐ and 3.5‐fold increase in steady‐state levels of procollagen I and III mRNA levels respectively, whereas l ‐ascorbate had no effect. Palmitoyl ascorbate and l ‐ascorbate induced similar increases in the amounts of newly synthesized procollagen secreted into the medium and in the amounts of collagen types I, III and V accumulating in the cell layer. There was no effect of either palmitoyl ascorbate or l ‐ascorbate on the activity of a procollagen α 2 (I) promoter construct transiently transfected into HISM cells. Palmitoyl ascorbate augments HISM cell procollagen synthesis and mRNA levels more efficiently than l ‐ascorbate. This property may be due to the greater resistance of the ascorbate ester to oxidation and suggests that palmitoyl ascorbate could be an important agent for studies of collagen synthesis in vitro. J. Cell. Biochem. 73:312–320, 1999. © 1999 Wiley‐Liss, Inc.