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Characterization of the promoter for the human antisense fibroblast growth factor‐2 gene; Regulation by Ets in Jurkat T cells
Author(s) -
Gag Michael L.,
Moy Grace K.,
Klagsbrun Michael
Publication year - 1999
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19990315)72:4<492::aid-jcb5>3.0.co;2-h
Subject(s) - jurkat cells , microbiology and biotechnology , biology , exon , antisense rna , transfection , sense (electronics) , untranslated region , open reading frame , promoter , gene , enhancer , complementary dna , gene expression , messenger rna , genetics , t cell , chemistry , peptide sequence , immune system
Human lymphoid cells were found to synthesize predominantly antisense, and not sense, fibroblast growth factor‐2 (FGF‐2) mRNA. Two cDNAs corresponding to human 1069‐ and 1173‐nucleotide antisense FGF‐2 mRNAs were cloned from Jurkat T cells. The two cDNAs each possess a unique exon 1 and common exon 2, 3, 4, and 5 sequences. Exon 4 and 5 sequences overlap in the 3′ untranslated region of FGF‐2 cDNA, but not in the FGF‐2 open reading frame. This is unlike the Xenopus antisense FGF‐2 homologue, which overlaps with parts of both the FGF‐2 3′ untranslated region and its open reading frame. To investigate the regulation of human antisense FGF‐2 gene expression, a 2.5‐kilobase (kb) promoter region was isolated and characterized. Transient transfection of promoter‐luciferase constructs demonstrated the antisense FGF‐2 promoter to be active in Jurkat cells. Using transient transfection and in vitro binding assays, specific mutations within the promoter sequence have implicated that Ets‐like transcription factors are significant in regulating the human antisense FGF‐2 gene in Jurkat cells. J. Cell. Biochem. 72:492–506, 1999. © 1999 Wiley‐Liss, Inc.