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Divergent regulation of 1,25‐dihydroxyvitamin D 3 on human bone marrow osteoclastogenesis and myelopoiesis
Author(s) -
Qi Dan Yi,
Perkins Sherrie L.,
Kling Stephen J.,
Russell R. Graham G.
Publication year - 1999
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19990301)72:3<387::aid-jcb8>3.0.co;2-7
Subject(s) - myelopoiesis , myeloid , clonogenic assay , haematopoiesis , colony stimulating factor , endocrinology , biology , medicine , osteoclast , bone marrow , cell culture , cancer research , microbiology and biotechnology , immunology , biochemistry , stem cell , in vitro , genetics
The physiologically active form of vitamin D 3 , 1,25‐dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) has influence over osteoclastogenesis and myelopoiesis, but the regulational mechanism is not well‐defined. In this report, formation of osteoclast‐like (OCL) cells from primitive myeloid colony‐forming cells (PM‐CFC) as mediated by 1,25(OH) 2 D 3 was examined. Our results present in this report clearly show that 1,25(OH) 2 D 3 dose‐dependently stimulated OCL cell formation when added to suspension cultures of individually replated PM‐CFC colonies. Marrow cells were plated with either granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) or the human bladder carcinoma cell line 5637 conditioned medium (5637 CM) as the source of colony‐stimulating activity. The 1,25(OH) 2 D 3 effect of osteoclast differentiation was associated with a concomitant decrease in clonogenic growth of myelopoietic progenitors in response to colony‐stimulating activity. Secondly, the effect of adding the known stimulator of hematopoiesis, interleukin‐1β (IL‐1β) and/or 1,25(OH) 2 D 3 on human myeloid colony growth was assessed. IL‐1β enhanced the formation of primitive myeloid colonies in response to GM‐CSF by 160%. On the other hand, 1,25(OH) 2 D 3 dose‐dependently inhibited both GM‐CSF‐ and 5637 CM‐driven myeloid colony formation by as much as 90% at 100 nM. Addition of IL‐1β to GM‐CSF‐stimulated cultures dampened the inhibitory effect of 1,25(OH) 2 D 3 . The inhibition of myeloid clonogenic growth by 1,25(OH) 2 D 3 was almost abolished (89%) by simultaneously adding anti‐tumor necrosis factor‐α monoclonal antibody (anti‐TNF‐α MoAb) to the culture medium. These results collectively suggest divergent roles for 1,25(OH) 2 D 3 in osteoclastogenesis and myelopoiesis, promoting the differentiation of OCL cells from primitive myeloid cells but inhibiting the proliferation of later myeloid progenitor cells. This inhibition of myeloid progenitors may be mediated by TNF‐α. J. Cell. Biochem. 72:387–395, 1999. © 1999 Wiley‐Liss, Inc.