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Interbands of Drosophila melanogaster polytene chromosomes contain matrix association regions
Author(s) -
Schwartz Yu.B.,
Ioudinkova E.S.,
Demakov S.A.,
Razin S.V.,
Zhimulev I.F.
Publication year - 1999
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19990301)72:3<368::aid-jcb6>3.0.co;2-c
Subject(s) - polytene chromosome , drosophila melanogaster , biology , genetics , melanogaster , interphase , matrix (chemical analysis) , dna , microbiology and biotechnology , gene , chemistry , chromatography
The DNA of three previously cloned interband regions (85D9/D10, 86B4/B6, and 61C7/C8) of Drosophila melanogaster polytene chromosomes has been tested for the presence of matrix association regions (MAR), using the in vitro matrix‐binding assay of Cockerill and Garrard. MARs were found in all three interband regions under study. These results are discussed in frames of a model postulating that interband regions of polytene chromosomes correspond to the chromosomal DNA loop borders, which can be identified in interphase nuclei using biochemical approaches. J. Cell. Biochem. 72:368–372, 1999. © 1999 Wiley‐Liss, Inc.