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Effects of prolactin on aldosterone secretion in rat zona glomerulosa cells
Author(s) -
Kau MeiMei,
Lo MingJae,
Tsai ShiowChwen,
Chen JiannJong,
Pu HsiaoFung,
Chien Eileen Jea,
Chang LingLing,
Wang Paulus S.
Publication year - 1999
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19990201)72:2<286::aid-jcb13>3.0.co;2-4
Subject(s) - aldosterone , medicine , endocrinology , zona glomerulosa , secretion , corticosterone , pregnenolone , chemistry , egta , angiotensin ii , mineralocorticoid , biology , hormone , steroid , calcium , receptor
Acute effects and action mechanisms of prolactin (PRL) on aldosterone secretion in zona glomerulosa (ZG) cells were investigated in ovariectomized rats. Administration of ovine PRL (oPRL) increased aldosterone secretion in a dose‐dependent manner. Incubation of [ 3 H]‐pregnenolone combined with oPRL increased the production of [ 3 H]‐aldosterone and [ 3 H]‐deoxycorticosterone but decreased the accumulation of [ 3 H]‐corticosterone. Administration of oPRL produced a marked increase of adenosine 3′,5′‐cyclic monophosphate (cAMP) accumulation in ZG cells. The stimulatory effect of oPRL on aldosterone secretion was attenuated by the administration of angiotensin II (Ang II) and high potassium. The Ca 2+ chelator, ethylene glycol‐bis(β‐aminoethyl ether)‐N,N,N′,N′‐tetraacetic acid (EGTA, 10 −2 M), inhibited the basal release of aldosterone and completely suppressed the stimulatory effects of oPRL on aldosterone secretion. The stimulatory effects of oPRL on aldosterone secretion were attenuated by the administration of nifedipine (L‐type Ca 2+ channel blocker) and tetrandrine (T‐type Ca 2+ channel blocker). These data suggest that the increase of aldosterone secretion by oPRL is in part due to (1) the increase of cAMP production, (2) the activation of both L‐ and T‐type Ca 2+ channels, and (3) the activation of 21‐hydroxylase and aldosterone synthase in rat ZG cells. J. Cell. Biochem. 72:286–293, 1999. © 1999 Wiley‐Liss, Inc.