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Application of magnetic field–induced heat shock protein 70 for presurgical cytoprotection
Author(s) -
Han Li,
Lin Hana,
Head Mark,
Jin Ming,
Blank Martin,
Goodman Reba
Publication year - 1998
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19981215)71:4<577::aid-jcb12>3.0.co;2-v
Subject(s) - cytoprotection , hsp70 , heat shock protein , shock (circulatory) , medicine , chemistry , biochemistry , oxidative stress , gene
To develop an alternative to hyperthermia for the induction of hsp70 for presurgical cytoprotection, we investigated the optimal exposure conditions for magnetic field induction of hsp70. Normal human breast cells (HTB124) were exposed to 60‐Hz magnetic fields and hsp70 levels were measured following three different exposure conditions: continuous exposure up to 3 h, a single 20‐min exposure, and a single 20‐min exposure followed by repeated 20‐min exposures at different field strengths. In cells exposed continuously for 3 h, hsp70 levels peaked (46%) within 20 min and returned to control levels by 2 h. Following a single 20‐min exposure, the return of hsp70 levels to control values extended to more than 3 h. When cells underwent a 20‐min exposure followed by repeated 20‐min exposures (restimulation) with different field strengths, additional increases in hsp70 levels were induced: 31% at 1 h, 41% at 2 h, and 30% at 3 h. J. Cell. Biochem. 71:577–583, 1998. © 1998 Wiley‐Liss, Inc.