Premium
Angiotensin II induces diverse signal transduction pathways via both G q and G i proteins in liver epithelial cells
Author(s) -
Tsygankova Oxana M.,
Peng Ming,
Maloney Judith A.,
Hopkins Nancy,
Williamson John R.
Publication year - 1998
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19980401)69:1<63::aid-jcb7>3.0.co;2-t
Subject(s) - gq alpha subunit , microbiology and biotechnology , signal transduction , angiotensin ii , g protein , chemistry , biology , receptor , biochemistry
Angiotensin II stimulates a biphasic activation of Raf‐1, MEK, and ERK in WB liver epithelial cells. The first peak of activity is rapid and transient and is followed by a sustained phase. Angiotensin II also causes a rapid activation of p21 ras in these cells. Moreover, two Src family kinases (Fyn and Yes) were activated by angiotensin II in a time‐ and concentration‐dependent manner. Microinjection of antibodies against Fyn and Yes blocked angiotensin II‐induced DNA synthesis and c‐Fos expression in WB cells, indicating an obligatory involvement of these tyrosine kinases in the activation of the ERK cascade by angiotensin II. Finally, substantial reduction of the angiotensin II‐stimulated activation of Fyn, Raf‐1, ERK, and expression of c‐Fos by pertussis toxin pretreatment argues that G proteins of the G i family as well as the G q family are involved in angiotensin II‐mediated mitogenic pathways in WB cells. J. Cell. Biochem. 69:63–71, 1998. © 1998 Wiley‐Liss, Inc.