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DNA loop anchorage region colocalizes with the replication origin located downstream to the human gene encoding lamin B2
Author(s) -
Lagarkova Maria A.,
Svetlova Ekaterina,
Giacca Mauro,
Falaschi Arturo,
Razin Sergey V.
Publication year - 1998
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19980401)69:1<13::aid-jcb2>3.0.co;2-y
Subject(s) - biology , gene , dna replication , genetics , topoisomerase , microbiology and biotechnology , dna , origin recognition complex , eukaryotic dna replication
The recently developed procedure of topoisomerase II‐mediated DNA loop excision has been used to analyze the topological organization of a human genome fragment containing the gene encoding lamin B2 and the ppv1 gene. A 3.5 kb long DNA loop anchorage/topoisomerase II cleavage region was found within the area under study. This region includes the end of the lamin B2 coding unit and an intergenic region where an origin of DNA replication was previously found. These observations further corroborate the hypothesis that DNA replication origins are located at or close to DNA loop anchorage regions. J. Cell. Biochem. 69:13–18, 1998. © 1998 Wiley‐Liss, Inc.