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Developmental regulation of 14‐3‐3 ϵ isoform in rat heart
Author(s) -
Luk Sharon C.W.,
Ngai Saiming,
Tsui Stephen K.W.,
Chan Kwokkeung,
Fung Kwokpui,
Lee Cheukyu,
Waye Mary M.Y.
Publication year - 1998
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19980201)68:2<195::aid-jcb6>3.0.co;2-q
Subject(s) - complementary dna , northern blot , gene isoform , microbiology and biotechnology , biology , recombinant dna , messenger rna , cdna library , blot , clone (java method) , western blot , southern blot , gene expression , escherichia coli , gene , biochemistry
Human heart cDNA sequencing yielded a cDNA clone that is similar in DNA and amino acid sequences to that of mouse 14‐3‐3 ϵ isoform. The 6xHis‐tagged H1433ϵ recombinant protein was expressed in Escherichia coli and its size was approximately 30 kDa. From Northern blot results with human multiple tissues, human skeletal muscle was found to have the highest level of h1433ϵ mRNA expression, whereas Northern blots of human cancer cell lines detected the highest mRNA level of h1433ϵ in colorectal adenocarcinoma SW480. The protein expression level of h1433ϵ and Raf‐1 is found to be regulated coordinately during rat heart development, and their protein expression was highest from 14.5 to 16.5 days postcoitum. J. Cell. Biochem. 68:195–199, 1998. © 1998 Wiley‐Liss, Inc.