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Inducible expression of a mutant form of MEK1 in Swiss 3T3 cells
Author(s) -
Syu LiJyun,
Guan KunLiang,
Saltiel Alan R.
Publication year - 1997
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19971201)67:3<367::aid-jcb8>3.0.co;2-u
Subject(s) - mapk/erk pathway , mutant , kinase , map kinase kinase kinase , mitogen activated protein kinase kinase , mitogen activated protein kinase , phosphorylation , microbiology and biotechnology , protein kinase a , map2k7 , chemistry , c raf , cyclin dependent kinase 2 , biology , biochemistry , gene
We conditionally overexpressed a MEK1 mutant that contains triple mutations in the regulatory and kinase domains, and investigated its effects on the MAP kinase cascade in Swiss 3T3 cells. Expression of the mutant produced a 60% blockade in MAP kinase activity. However, only a modest blockade in DNA synthesis was observed, without any reductions in the phosphorylation of two proteins known to be substrates of MAP kinase. Moreover, the overexpression of MEK1(3A) failed to block endogenous MEK1 activation, although MEK1(3A) formed complexes with both c‐Raf and B‐Raf as well as p42/p44 MAPK. These results suggest that there may be multiple biochemical inputs into the MEK/MAPK pathway. J. Cell. Biochem. 67:367–377, 1997. © 1997 Wiley‐Liss, Inc.