Premium
Inhibition of osteoblastic cell differentiation by conditioned medium derived from the human prostatic cancer cell line PC‐3 in vitro
Author(s) -
Kido Junichi,
Yamauchi Noriyuki,
Ohishi Keiji,
Kataoka Masatoshi,
Nishikawa Seiji,
Nakamura Teruo,
Kadono Hiroyuki,
Ikedo Dai,
Ueno Akemichi,
omura Norio,
Okuyama Akihiko,
Nagata Toshihiko
Publication year - 1997
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19971101)67:2<248::aid-jcb10>3.0.co;2-b
Subject(s) - alkaline phosphatase , osteopontin , osteocalcin , cell culture , bone metastasis , cellular differentiation , chemistry , cancer cell , cell growth , osteoblast , cell , endocrinology , cancer research , medicine , biology , metastasis , in vitro , cancer , biochemistry , enzyme , genetics , gene
Human prostatic carcinoma frequently metastasizes to bone tissue and activates bone metabolism, especially bone formation, at the site of metastasis. It has been reported that an extract of prostatic carcinoma and conditioned medium (CM) of a human prostatic carcinoma cell line, PC‐3, established from a bone metastastic lesion, stimulate osteoblastic cell proliferation. However, there is little information about the effect of PC‐3 CM on the differentiation of osteoblastic cells. In this study, we investigated the effect of PC‐3 CM on the differentiation of two types of osteoblastic cells, primary fetal rat calvaria (RC) cells containing many undifferentiated osteoprogenitor cells, and ROS 17/2.8, a well‐differentiated rat osteosarcoma cell line. PC‐3 CM inhibited bone nodule formation and the activity of alkaline phosphatase (ALPase), an osteoblastic marker enzyme, on days 7, 14, and 21 (RC cells) or 3, 6, and 9 (ROS 17/2.8 cells) in a dose‐dependent manner (5–30% CM). However, the CM did not affect cell proliferation or cell viability. PC‐3 CM was found to markedly block the gene expression of ALPase and osteocalcin (OCN) mRNAs but had no effect on the mRNA expression of osteopontin (OPN), the latter two being noncollagenous proteins related to bone matrix mineralization. These findings suggest that PC‐3 CM contains a factor that inhibits osteoblastic cell differentiation and that this factor may be involved in the process of bone metastasis from prostatic carcinoma. J. Cell. Biochem. 67:248–256, 1997. © 1997 Wiley‐Liss, Inc.