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Subcellular localization of G‐proteins in primary‐cultured mouse preadipocytes and adipocytes
Author(s) -
BéginHeick Nicole,
Black Marsha A.,
Gaffield Johanna,
Cadrin Monique
Publication year - 1997
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(199705)65:2<259::aid-jcb11>3.0.co;2-d
Subject(s) - colocalization , subcellular localization , intracellular , microbiology and biotechnology , cytoplasm , protein subcellular localization prediction , adipose tissue , biology , membrane protein , immunoprecipitation , cell culture , chemistry , membrane , biochemistry , gene , genetics
The subcellular localization of G s α, G i α1&2, G i α3, and Gβ was studied in primary‐cultured undifferentiated and differentiated, lipid replete, adipose cells. The results show a distinct distribution for each of these G‐proteins and differences between differentiated and undifferentiated cells. All the G‐proteins examined had a cytoplasmic localization; only G i α1 and 2 showed a significant colocalization with the plasma membrane and this only in differentiated cells. Most studies using cells in culture have reported an intracellular localization for G‐proteins, whereas in tissue sections the localization has been reported to be largely with the plasma membrane, with some intracellular localization. The results suggest that the cell‐cell interactions or the specific geometry imposed by culture conditions favor the intracellular compared to peripheral localization of G‐proteins. Alternately, the posttranslational modifications necessary for G‐protein insertion in the plasma membrane may be deficient in cultured cells. J. Cell. Biochem. 65:259–266. © 1997 Wiley‐Liss, Inc.