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Dual transduction signaling by a Xenopus muscarinic receptor: Adenylyl cyclase inhibition and MAP kinase activation
Author(s) -
Herrera Luisa,
Hinrichs María Victoria,
Frías Jorge,
Gutkind Silvio,
Olate Juan
Publication year - 1997
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(199704)65:1<75::aid-jcb8>3.0.co;2-w
Subject(s) - muscarinic acetylcholine receptor , muscarinic acetylcholine receptor m5 , adenylyl cyclase , microbiology and biotechnology , muscarinic acetylcholine receptor m2 , signal transduction , mapk/erk pathway , chemistry , muscarinic acetylcholine receptor m1 , protein kinase a , biology , muscarinic acetylcholine receptor m3 , receptor , kinase , biochemistry
Using transient transfection of COS‐7 and human embryonic kidney 293 cells, we studied the functional properties of a previously cloned muscarinic Xenopus receptor [Herrera et al. (1994): FEBS Lett 352:175–179] and its coupling to adenylyl cyclase (AC) and mitogen‐activated protein kinase (MAPK) pathways. Expression of the Xenopus muscarinic receptor results in the inhibition of AC activity and activation of the MAPK pathway through a mechanism that involves a Pertussis‐sensitive G‐protein and the Gβγ subunits. The signal transduction properties of this receptor are similar to the mammalian m2 and m4 muscarinic receptors. These results strongly support the idea that inhibition of AC and MAPK activation, signaled out from the muscarinic oocyte receptor, are involved in the oocyte maturation process. J. Cell. Biochem. 65:75–82. © 1997 Wiley‐Liss, Inc.