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Retinoic acid stimulates growth hormone synthesis in human somatotrophic adenoma cells: Characterization of its nuclear receptors
Author(s) -
Guibourdenche Jean,
Djakouré Charlotte,
Porquet Dominique,
Pagésy Patrick,
RochetteEgly Cécile,
Peillon Françoise,
Yuan Li Jacques,
EvainBrion Danièle
Publication year - 1997
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(199704)65:1<25::aid-jcb3>3.0.co;2-0
Subject(s) - retinoic acid , receptor , chemistry , nuclear receptor , retinoid x receptor gamma , medicine , growth hormone , adenoma , endocrinology , retinoic acid inducible orphan g protein coupled receptor , microbiology and biotechnology , hormone , biology , biochemistry , retinoic acid receptor , gene , transcription factor
In order to gain a better understanding on the possible role of retinoic acid (RA) on human GH secretion, we have characterized the expression of its nuclear receptors in somatotropic adenoma cell extracts. By immunoblotting with rabbit polyclonal antibodies directed against RARα, β, and γ and RXRα and β, we could only detect the presence of RARα and RXRα proteins. The predominant expression of RXRα was confirmed at the mRNA level by Northern and slot‐blot analysis. When then investigated the effect of RA on GH synthesis in cell culture of adenomatous somatotrophs. In cultured cells, RA (1 μM) stimulated GH secretion, increased intracellular GH content and GH mRNA levels within 72 h, suggesting a modulation of GH synthesis by RA. J. Cell. Biochem 65:25–31. © 1997 Wiley‐Liss, Inc.