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Fas‐induced changes in cdc2 and cdk2 kinase activity are not sufficient for triggering apoptosis in HUT‐78 cells
Author(s) -
De Luca Antonio,
De Maria Ruggero,
Baldi Alfonso,
Trotta Rossana,
Facchiano Francesco,
Giordano Antonio,
Testi Roberto,
Condorelli Gianluigi
Publication year - 1997
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19970315)64:4<579::aid-jcb6>3.0.co;2-o
Subject(s) - cyclin dependent kinase 1 , cyclin dependent kinase 2 , apoptosis , programmed cell death , kinase , microbiology and biotechnology , fas ligand , biology , cell cycle , cancer research , chemistry , protein kinase a , biochemistry
Recent evidence suggested a role for the cell cycle dependent kinases cdc2 and cdk2 in apoptosis. An important mechanism by which many cell types could undergo apoptosis is through the activation of the Fas molecule on the cell membrane. To investigate whether Fas‐induced cell death activated cdc2 and cdk2 kinases inappropriately, the human T lymphoma cells HUT‐78, which express a high copy number of Fas, and two other previously characterized subclones of the same cell line which express mutant, cell death‐deficient dominant‐negative forms of Fas, were Fas‐challenged and the changes in cdc2 and cdk2 kinase activity monitored. In both wild‐type and Fas‐mutated HUT‐78 cells, apoptosis was associated simultaneously with decreased cdc2 and increased cdk2 activity. This association suggested that changes in cdc2 and cdk2 kinase activity are secondary events in cell death mediated by Fas. J. Cell. Biochem. 64:579–585. © 1997 Wiley‐Liss, Inc.