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Influence of mechanical activity, adrenergic stimulation, and calcium on the expression of myosin heavy chains in cultivated neonatal cardiomyocytes
Author(s) -
Luther Hans Peter,
Hille Simone,
Haase Hannelore,
Morano Ingo
Publication year - 1997
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19970301)64:3<458::aid-jcb12>3.0.co;2-c
Subject(s) - myosin , stimulation , calcium , adrenergic , myosin light chain kinase , biophysics , medicine , microbiology and biotechnology , chemistry , biology , receptor
It is generally accepted that mechanical stress of cardiomyocytes increases RNA and protein synthesis of myosin heavy chain (MHC) quantitatively but it is still a matter of debate whether MHC gene expression is also changed qualitatively. We investigated expression of MHC genes of spontaneously contracting neonatal cardiomyocytes experimentally arrested by permanent depolarization [potassium chloride (KCI)] as well as by electromechanical uncoupling [2,3 butanedione monoxime (BDM)]. Relative distribution of MHC mRNA isoforms (α and β) was studied by quantitative polymerase chain reaction. Expression of MHC isoenzymes was the same in contracting (34.5% β‐MHC) and arrested (40.5% and 33.0% β‐MHC in KCl and BDM, respectively) cardiomyocytes. However, treatment with phenylephrine for the same period increased significantly β‐MHC expression to 55%. We conclude that hormonal factors rather than Ca 2− or mechanical stress regulate qualitatively MHC gene expression. J. Cell. Biochem. 64:458–465. © 1997 Wiley‐Liss, Inc.