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Regulation of c‐ fos is affected by electromagnetic fields
Author(s) -
Rao Sharmila,
Henderson Ann S.
Publication year - 1996
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19961201)63:3<358::aid-jcb11>3.0.co;2-d
Subject(s) - transfection , hela , plasmid , reporter gene , microbiology and biotechnology , gene , biology , dna , regulatory sequence , gene expression , electromagnetic field , chemistry , cell culture , genetics , physics , quantum mechanics
The goal of the present study was to determine if regulatory regions of the c‐ fos gene were responsive to electromagnetic field exposure. The research design used transfected cells to increase the sensitivity of assays designed to identify changes following exposure. HeLa cells were transiently transfected with plasmids containing upstream regulating regions of c‐ fos up to ‐700 base pairs, coupled with the prokaryotic reporter gene CAT. Cells were exposed to an environmentally relevant EMF of 60 Hz at 60 mG rms . CAT expression above control levels in transfected cells (region +42 to ‐700 bp) was observed following 5 min exposure to the electromagnetic field, with a peak at 20 min. The expression was at basal levels following 40 min exposure. Deletion analysis of upstream DNA narrowed the responsive region to 138 base pairs from ‐363 to ‐225, which contains the SRE/AP‐1 sites. © 1996 Wiley‐Liss, Inc.