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Dexamethasone regulation of marrow stromal‐derived osteoblastic cells
Author(s) -
Fried A.,
Benayahu D.
Publication year - 1996
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19960915)62:4<476::aid-jcb5>3.0.co;2-j
Subject(s) - dexamethasone , stromal cell , matrigel , alkaline phosphatase , chemistry , glucocorticoid , endocrinology , medicine , microbiology and biotechnology , cell , biology , enzyme , biochemistry
The clonal subtypes of cells in the osteogenic family represented by fibroblastoid MBA‐15.33, preosteoblast MBA‐15.4, and mature osteoblastic MBA‐15.6 cells were used to study the effects of glucocorticoid (dexamethasone). The role of dexamethasone was monitored on cell attachment when plated on various protein substrata (BSA, collagen I, and Matrigel). A 24 h exposure of the cells to 10 ‐6 M or 10 ‐7 M dexamethasone differential affects their attachment preference. MBA‐15.33 and MBA‐15.4 cells increased their attachment capability on collagen I, while MBA‐15.6 cells' attachment was inhibited. Pretreatment with (10 ‐6 M) dexamethasone caused an increase in attachment on Matrigel by MBA‐15.33 cells and to less extent by MBA‐15.4 cells. Additionally, measurements of two enzymatic activities were monitored; one is alkaline phosphatase (ALK‐P), and the second is neutral endopeptidase (CD10/NEP). MBA‐15.33, MBA‐15.4, and MBA‐15.6 cells were exposed to dexamethasone or to various growth factors (bone morphogenic protein (BMP‐2 and BMP‐3), TGFβ, and IGF‐I). In some experiments, pretreatment of cells by dexamethasone was followed by exposure to the growth factors. The cells' challenged cellular responses were not uniform and revealed a differential pattern when their ALK‐P and CD10/NEP enzymatic activities were measured. © 1996 Wiley‐Liss, Inc.