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Simultaneous digital imaging analysis of cytosolic calcium and morphological change in platelets activated by surface contact
Author(s) -
Ikeda Masataka,
Ariyoshi Hideo,
Kambayashi Junichi,
Sakon Masato,
Kawasaki Tomio,
Monden Morito
Publication year - 1996
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19960501)61:2<292::aid-jcb12>3.0.co;2-o
Subject(s) - platelet , pseudopodia , differential interference contrast microscopy , biophysics , chemistry , confocal , cytoplasm , confocal laser scanning microscopy , confocal microscopy , microscopy , cell , optics , microbiology and biotechnology , biochemistry , biology , physics , immunology
The dynamic change of cytoplasmic Ca 2+ concentration ([Ca 2+ ] i ) and morphological change were investigated simultaneously by confocal laser scanning microscopy using fluo‐3 and by differential interference contrast optics in platelets activated by contact with the following types of surfaces: native glass and glass treated with poly‐ L ‐lysine (PLL), fibrinogen (Fg), or von Willebrand factor (vWF). The initial [Ca 2+ ] i values just after the surface contact were comparable (approximately 100 nM) among platelets deposited on the four surface types. On the PLL‐surface, no morphological change or [Ca 2+ ] i elevation was observed. Glass‐, Fg‐, and vWF‐surface adhered platelets showed pseudopod formation and spreading associated with the inhomogeneous [Ca 2+ ] i rise. The platelets on the Fg‐surface were the most active in terms of [Ca 2+ ] i rise and morphological change. During pseudopod formation, the mean [Ca 2+ ] i value was maximal and localized high [Ca 2+ ] i zones were observed inside pseudopods, as well as in the center of the platelets. After spreading, high [Ca 2+ ] i zones still remained in the center of the cell. This new technique enabled simultaneous observation of [Ca 2+ ] i and cell shape and we clearly demonstrated a close relationship between [Ca 2+ ] i and morphological alterations. © 1996 Wiley‐Liss, Inc.