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Identification and characterization of various differentiative growth plate chondrocytes from porcine by countercurrent centrifugal elutriation
Author(s) -
Lee K.M.,
Fung K.P.,
Leung P.C.,
Leung K.S.
Publication year - 1996
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19960315)60:4<508::aid-jcb7>3.0.co;2-w
Subject(s) - elutriation , alkaline phosphatase , chondrocyte , proteoglycan , biology , chemistry , dna synthesis , microbiology and biotechnology , biochemistry , dna , in vitro , enzyme , extracellular matrix , organic chemistry
Countercurrent centrifugal elutriation was used to separate growth plate chondrocytes from porcine basing on their differences in sizes and densities. Eighteen fractions of cells with different sizes and densities were obtained. The mean cellular volumes increased progressively in each of successive fractions, and that increase was associated with specific phenotypic changes, such as biochemical differences in DNA synthesis, proteoglycan synthesis, and activities of alkaline phosphatase. Three distinct chondrocyte subpopulations with their unique characteristics were identified among the elutriated fractions. The resting chondrocytes were found to be small in size and quiescent. The hypertrophic chondrocytes were found to be large in size and metabolically active both in alkaline phosphatase and in proteoglycan productions. The proliferative chondrocytes exhibited a high DNA synthesis rate, and their sizes were found to be between those of the resting and hypertrophic chondrocytes. © 1996 Wiley‐Liss, Inc.