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Transcriptional downregulation of stromelysin by tetracycline
Author(s) -
Jonat Carsten,
Chung FuZon,
Baragi Vijaykumar M.
Publication year - 1996
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19960301)60:3<341::aid-jcb6>3.0.co;2-w
Subject(s) - chloramphenicol acetyltransferase , tetracycline , psychological repression , downregulation and upregulation , microbiology and biotechnology , chemistry , transcriptional regulation , matrix metalloproteinase , transfection , gene expression , biology , reporter gene , gene , biochemistry , antibiotics
We investigated the role of tetracycline in the transcriptional regulation of matrix metalloproteinases. Using interleukin‐1β (IL‐1) induced stromelysin as a model system, we describe the repression of the endogenous stromelysin RNA accumulation, as well as the transcriptional inhibition of various stromelysin promoter/chloramphenicol‐acetyltransferase constructs in transient transfection assays. The inhibition occurred in a dose‐dependent fashion, with an IC 50 of about 1 μM. Our results suggest that the transcriptional inhibition by tetracycline is not due to a block of activity of the activating protein complex 1 (AP‐1) but is mediated by sequences upstream of the AP‐1 binding site. © 1996 Wiley‐Liss, Inc.