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Genes that regulate apoptosis in the mouse thymus
Author(s) -
Osborne Barbara A.,
Smith Sallie W.,
McLaughlin Kelly A.,
Grimm Lisa,
Kallinch Tilmann,
Liu Zhenggang,
Schwartz Lawrence M.
Publication year - 1996
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(19960101)60:1<18::aid-jcb4>3.0.co;2-6
Subject(s) - apoptosis , microbiology and biotechnology , gene , biology , genetics
Abstract Elimination of self‐reactive T lymphocytes occurs during T‐cell development in the thymus by a process known as negative selection. The mechanism that drives negative selection is apoptosis. To identify genes that regulate apoptosis in the mouse thymus, a library of negatively selected T cells was constructed and, by subtractive screening, several differentially regulated genes were isolated. Two transcripts that are repressed during cell death were identified, in addition to two induced transcripts. Further experiments demonstrated that cell death in thymocytes can occur via several induction pathways and each pathway appears to be regulated by a unique cascade of genes. © 1996 Wiley‐Liss, Inc.