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Mutational specificity and cancer chemoprevention
Author(s) -
Curry John,
Khaidakov Mohammed,
da Cruz Aparecido,
Karnaoukhova Larissa,
Kusser Wolfgang C.,
de Boer Johan,
Moffat Joyce,
Glickman Barry W.
Publication year - 1996
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/(sici)1097-4644(1996)25+<99::aid-jcb14>3.0.co;2-i
Subject(s) - frameshift mutation , genetics , biology , mutagen , mutation , context (archaeology) , mutagenesis , population , computational biology , dna , gene , medicine , paleontology , environmental health
Mutational specificity describes the composite of all of the genetic alterations in a collection of mutations arising from a specific treatment. The information includes not only the nature of the genetic change (e.g., a base substitution or a frameshift), but also information about nucleotide position and hence the DNA context. As both the type of DNA damage and its position can be expected to reflect the nature of the chemical and physical mutagen, mutational specificity can be expected to provide insights into mechanisms of mutation. Conversely, mutational spectra should also provide insights into the identity of the mutagen. Indeed, the pioneering work on mutational specificity in Escherichia coli indicates that each physical or chemical treatment produces a unique spectrum of mutations. With the application of biotechnology to the field of genotoxicology, the database of sequenced mutations has become quite substantial. Both in vitro and in vivo data has been obtained following exposure to a variety of agents. In this communication we will critically assess whether the reality of mutational specificity has fulfilled the expectations and to examine what potential remains to be explored, especially in the area of monitoring human populations. The usefulness of both mutational spectra analysis and population monitoring with regards to chemoprevention are discussed. J. Cell. Biochem. 25S:99–107. © 1997 Wiley‐Liss, Inc.