Premium
Synthesis of peptides onto the surface of poly(ethylene terephthalate) particle track membranes
Author(s) -
Papra Alexander,
Hicke HansGeorg,
Paul Dieter
Publication year - 1999
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19991114)74:7<1669::aid-app9>3.0.co;2-w
Subject(s) - surface modification , polymer chemistry , membrane , carbodiimide , particle (ecology) , x ray photoelectron spectroscopy , materials science , ethylene , coupling reaction , poly ethylene , chemical engineering , chemistry , organic chemistry , catalysis , biochemistry , oceanography , engineering , geology
In this article, we describe the synthesis of polypeptides onto the whole specific surface of poly(ethylene terephthalate) particle track membranes. In this case, initial functionalization is necessary. It had been done by an optimized surface oxidation in accordance with the method of Marchant‐Brynaert, J.; Deldime, M.; Dupont, I.; Dewez, J.‐L.; Schneider, Y.‐J. (J Colloid Interface Sci 1995, 173, 236). Subsequent activation with carbodiimide and reaction with multifunctional amines yielded an aminated surface. The synthesis of peptides was carried out by two different means: first, by the coupling of single amino acids analogous to the method of Merrifield, and second, by coupling of presynthesized polypeptides consisting of 18 helix‐forming amino acids. Analytical characterization was carried out by fluorescence spectroscopy by using the label Fluram® and confirmed by the results of X‐ray photoelectron (XP) spectroscopy. Only the stepwise synthesis led to a dense surface functionalization with peptides, whereas the coupling of fragments resulted in lower yields of coupling. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 74: 1669–1674, 1999