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Synthesis and functionality of poly( N ‐vinylalkylamide). X. A novel aqueous two‐phase system based on thermosensitive polymers and dextran
Author(s) -
Kishida Akio,
Kikunaga Yoshihiro,
Akashi Mitsuru
Publication year - 1999
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19990923)73:13<2545::aid-app1>3.0.co;2-1
Subject(s) - polymer , dextran , aqueous solution , ethylene glycol , lower critical solution temperature , polymer chemistry , thermoresponsive polymers in chromatography , materials science , phase (matter) , chemical engineering , myoglobin , aqueous two phase system , chemistry , chromatography , copolymer , organic chemistry , reversed phase chromatography , engineering
The use of thermosensitive polymers in an aqueous two‐phase system was studied. Poly( N ‐isopropylacrylamide) (PNIPAAm) and poly( N ‐vinylisobutyramide) (PNVIBA) were used as thermosensitive polymers. Both polymers could form aqueous two‐phase with dextran, respectively. The phase diagrams of each system were successfully obtained. Using myoglobin as a model protein, a preliminary separation study was performed. The separation ability of both polymers was higher than that from the poly(ethylene glycol)‐dextran system. Protein separation ability appeared to be related to the hydrophilic/hydrophobic balance of the polymers. Both PNVIBA and PNIPAAm rich phases maintained their thermosensitivity after two‐phase formation. PNVIBA and PNIPAAm are useful as polymers for a functional aqueous two‐phase system. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 73: 2545–2548, 1999