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Relation between hydrophilicity and cell culturing on polystyrene Petri dish modified by ion‐assisted reaction
Author(s) -
Choi SungChang,
Choi WonKook,
Jung HungJin,
Park JungGyu,
Chung BongChul,
Yoo YoungSook,
Koh SeokKeun
Publication year - 1999
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19990705)73:1<41::aid-app5>3.0.co;2-3
Subject(s) - polystyrene , wetting , contact angle , petri dish , irradiation , materials science , x ray photoelectron spectroscopy , polymer chemistry , surface modification , chemical engineering , ion , oxygen , chemistry , polymer , organic chemistry , composite material , genetics , physics , nuclear physics , engineering , biology
Polystyrene Petri dishes were modified by an ion‐assisted reaction to supply a suitable surface for culturing cells. Wettability was measured by a contact anglometer after surface modification of polystyrene. Contact angles of water on the polystyrene were not reduced much by Ar + ion irradiation only, but dropped rapidly to a minimum of 19°, when polystyrene surface was modified by Ar + ion irradiation with flowing oxygen gas. X‐ray photoelectron spectroscopy analyses showed that hydrophilic groups were formed on the surface of polystyrene by a chemical reaction between unstable chains induced by the ion irradiation and the blown oxygen gas. Newly formed hydrophilic groups were identified as CO, (CO) and (CO)O bonds. The influence of the ion beam modification in growth of the rat pheochromocytoma cells was investigated. The results showed exclusively preferential cell growth in the polystyrene Petri dish that was treated by the ion‐assisted reaction. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 73: 41–46, 1999