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New metal chelate sorbent for albumin adsorption: Cibacron Blue F3GA‐Zn(II) attached microporous poly(HEMA) membranes
Author(s) -
Denizli Adil,
Salih Bekir,
Şenel Serap,
Arica M. Yakup
Publication year - 1998
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19980425)68:4<657::aid-app17>3.0.co;2-o
Subject(s) - membrane , adsorption , chemistry , bovine serum albumin , aqueous solution , desorption , nuclear chemistry , polymer chemistry , methacrylate , chelation , sorbent , metal ions in aqueous solution , inorganic chemistry , chromatography , metal , organic chemistry , copolymer , polymer , biochemistry
Poly(2‐hydroxyethyl methacrylate) [poly(HEMA)] membranes were prepared by UV‐initiated photopolymerization of HEMA in the presence of an initiator (α‐α′‐azobis‐isobutyronitrile, AIBN). The triazine dye Cibacron Blue F3GA was attached as an affinity ligand to poly(HEMA) membranes, covalently. These affinity membranes with a swelling ratio of 58% and containing 10.7 mmol Cibacron Blue F3GA/m 2 were used in the albumin adsorption studies. After dye‐attachment, Zn(II) ions were chelated within the membranes via attached‐dye molecules. Different amounts of Zn(II) ions [650–1440 mg Zn(II)/m 2 ] were loaded on the membranes by changing the initial concentration of Zn(II) ions and pH. Bovine serum albumin (BSA) adsorption on these membranes from aqueous solutions containing different amounts of BSA at different pH was investigated in batch reactors. The nonspecific adsorption of BSA on the poly(HEMA) membranes was negligible. Cibacron Blue F3GA attachment significantly increased the BSA adsorption up to 92.1 mg BSA/m 2 . Adsorption capacity was further increased when Zn(II) ions were attached (up to 144.8 mg BSA m 2 ). More than 90% of the adsorbed BSA was desorbed in 1 h in the desorption medium containing 0.5 M NaSCN at pH 8.0 and 0.025 M EDTA at pH 4.9. © 1998 John Wiley & Sons, Inc. J Appl Polym Sci 68: 657–664, 1998