Premium
Production and characterization of poly(ethylene glycol dimethacrylate‐styrene‐glycidyl methacrylate) microbeads
Author(s) -
Senel S.,
Cicek H.,
Tuncel A.
Publication year - 1998
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19980214)67:7<1319::aid-app23>3.0.co;2-v
Subject(s) - glycidyl methacrylate , ethylene glycol dimethacrylate , copolymer , suspension polymerization , polymer chemistry , monomer , styrene , materials science , methacrylate , benzoyl peroxide , polymerization , chemistry , methacrylic acid , polymer , composite material
Nonswellable and swellable poly(ethyleneglycol dimethacrylate)‐based microbeads that could react directly with the biological molecules were produced by a suspension polymerization procedure. For this purpose, ethyleneglycol dimethacrylate (EGDMA) was copolymerized with glycidyl methacrylate (GMA) in an aqueous suspension medium. Benzoyl peroxide and poly(vinyl alcohol) were used as the initiator and the stabilizer, respectively. The copolymerization provided nonswellable, tranparent, and spherical copolymer microbeads in the size range of 100–300 μm. On the other hand, swellable copolymer microbeads in the aqueous medium were obtained by using toluene as a diluent in the same copolymerization recipe. In a separate group of polymerizations, styrene (St) monomer was also included within the monomer phase to regulate the hydrophobicity of resulting microbeads. Nonswellable and swellable poly‐(EGDMA‐St‐GMA) microbeads were obtained by changing the type and concentration of the ingredients within the monomer phase. The effects of glycidyl methacrylate, styrene, and toluene concentrations on the microbead yield, the average size, and the swellability of microbeads were investigated. In the second part of the study, the interaction of produced microbeads with a selected enzyme (i.e., chymotrypsin) was investigated. The most stable chymotrypsin immobilization was achieved with the swellable poly(EGDMA)‐based microbeads including styrene. © 1998 John Wiley & Sons, Inc. J Appl Polym Sci 67:1319–1334, 1998