Premium
Structure and protein separation efficiency of poly( N ‐isopropylacrylamide) gels: Effect of synthesis conditions
Author(s) -
Kayaman Nilhan,
Kazan Dilek,
Erarslan Altan,
Okay Oğuz,
Baysal Bahattin M.
Publication year - 1998
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19980131)67:5<805::aid-app5>3.0.co;2-x
Subject(s) - poly(n isopropylacrylamide) , aqueous solution , polymer chemistry , copolymer , swelling , chemical engineering , polymerization , bovine serum albumin , chemistry , materials science , polymer , chromatography , organic chemistry , engineering
Crosslinked poly( N ‐isopropylacrylamide) (PNIPA) gels with different crosslink densities in the form of rods and beads were prepared by free‐radical crosslinking copolymerization. Solution and inverse suspension polymerization techniques were used for the gel synthesis. The gels were utilized to concentrate dilute aqueous solutions of penicillin G acylase (PGA), bovine serum albumin (BSA), and 6‐aminopenicillanic acid (6‐APA). The discontinuous volume transition at 34°C observed in the gel swelling was used as the basis of concentrating dilute aqueous protein solutions. PNIPA gels formed below 18°C were homogeneous, whereas those formed at higher temperatures exhibited heterogeneous structures. The water absorption capacity of PNIPA gels in the form of beads was much higher, and their rate of swelling was much faster than the rod‐shaped PNIPA gels. It was also found that the polymerization techniques used significantly affect the properties of PNIPA gels. The separation efficiency decreased when the protein molecules PGA or BSA in the external solution were replaced with small‐molecular‐weight compounds, such as 6‐APA. The protein separation efficiency by the gel beads increased to 100% after coating the bead surfaces with BSA. © 1998 John Wiley & Sons, Inc. J Appl Polym Sci 67: 805–814, 1998