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Synthesis and functionalities of poly( N ‐vinylalkylamide). VII. A novel aqueous two‐phase systems based on poly( N ‐vinylacetamide) and dextran
Author(s) -
Kishida Akio,
Nakano Shirou,
Kikunaga Yoshihiro,
Akashi Mitsuru
Publication year - 1998
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19980110)67:2<255::aid-app7>3.0.co;2-t
Subject(s) - dextran , myoglobin , peg ratio , ethylene glycol , chemistry , aqueous solution , phase (matter) , polymer chemistry , chemical engineering , chromatography , materials science , organic chemistry , finance , engineering , economics
It was found that a poly( N ‐vinylacetamide) (polyNVA)‐dextran system formed two phases. The top was polyNVA‐rich and the bottom phase was dextran‐rich, which is the same as the poly(ethylene glycol) (PEG)‐dextran system, and the phase separation occurred at a lower concentration than the PEG‐dextran system. The protein separation in the polyNVA‐dextran system was studied using myoglobin. Myoglobin was partitioned more in the bottom (dextran‐rich) phase in a polyNVA‐dextran system. The partition coefficient of the polyNVA‐dextran system was smaller that of the PEG‐dextran system, which suggests that the polyNVA‐dextran system is superior to the PEG‐dextran system for myoglobin separation. © 1998 John Wiley & Sons, Inc. J Appl Polym Sci 67: 255–258, 1998