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Preparation of biodegradable polymer microspheres encapsulating protein with micron sizes
Author(s) -
Li Xiongwei,
Xiao Jing,
Deng Xianmo,
Li Xiadyi,
Wang Hongli,
Jia Wenxiang,
Zhang Wenbing,
Men Li,
Yang Yan,
Zheng Zhenxi
Publication year - 1997
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19971017)66:3<583::aid-app20>3.0.co;2-v
Subject(s) - peg ratio , materials science , polyethylene glycol , polymer chemistry , polymer , differential scanning calorimetry , polyvinyl alcohol , ethylene glycol , chemical engineering , emulsion , copolymer , chemistry , organic chemistry , composite material , physics , finance , engineering , economics , thermodynamics
Biodegradable polymer poly‐ D,L ‐lactide‐polyethylene glycol (PLA‐PEG) was synthesized with stannous octoate (SnOc 2 ) as catalyst by a cationic ring‐opening polymerization. The molecular weight of PLA‐PEG is the highest at a content of 0.1% SnOc 2 . The PLA‐PEG microspheres carrying protein were prepared by a solvent evaporation composite emulsion technique with a narrow size range (1–2 μm). The sizes of PLA‐PEG microspheres increased with the increase of the molecular weight of PLA‐PEG. The PEG in PLA‐PEG (10%) significantly improved the size control of the microspheres of the PLA family as a drug carrier matrix. Polyvinyl alcohol (PVA) aqueous solution was used as dispersion medium for microsphere preparation. The concentration of the PVA solution can affect the size of the PLA‐PEG microspheres. The differential scanning calorimetry data showed that the PLA‐PEG microspheres can efficiently encapsulate the protein and that the crystalline of the microspheres carrying protein was lower than that of the nonprotein‐loading microspheres. The amount of protein carried in the PLA‐PEG microspheres was related to the nature of the protein itself. © 1997 John Wiley & Sons, Inc. J Appl Polym Sci 66: 583–590, 1997