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Covalent immobilization of nucleic acid probes onto reactive synthetic polymers
Author(s) -
Ferraton N.,
Delair Th.,
Laayoun A.,
Cros P.,
Mandrand B.
Publication year - 1997
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19971010)66:2<233::aid-app4>3.0.co;2-x
Subject(s) - covalent bond , nucleic acid , polymer , oligonucleotide , chemistry , polymer chemistry , hydrogen bond , reductive amination , amide , aldehyde , grafting , combinatorial chemistry , organic chemistry , molecule , dna , catalysis , biochemistry
Oligodeoxyribonucleotide (ODN)/polymer conjugates were obtained by covalent immobilization of various nucleic acid sequences to three different polymers. Two of them bore active ester groups for the covalent linkage via the formation of amide bonds and the third one featured aldehyde moieties for immobilization via reductive amination. The factors controlling the conjugation reaction were found to be the reactivities of the polymers and their abilities at forming hydrogen bonds with the ODNs to be tethered. It was found, as well, that the observed aggregation of the grafting reaction products was essentially due to hydrogen bonding with the nucleic bases of the tethered oligonucleotides. © 1997 John Wiley & Sons, Inc. J Appl Polym Sci 66: 233–242, 1997