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Congo red‐ and Zn(II)‐derivatized monosize poly(MMA‐HEMA) microspheres as specific sorbent in metal chelate affinity of albumin
Author(s) -
Denizli Adil,
Köktürk Gülay,
Salih Bekir,
Kozluca Ahmet,
Pişkin Erhan
Publication year - 1997
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19970103)63:1<27::aid-app5>3.0.co;2-u
Subject(s) - congo red , adsorption , nuclear chemistry , chemistry , aqueous solution , bovine serum albumin , desorption , metal ions in aqueous solution , chelation , sorbent , polymer , polymer chemistry , metal , inorganic chemistry , chromatography , organic chemistry
Monosize poly(methylmethacrylate‐hydroxyethylmethacrylate) [poly(MMA‐HEMA)] microspheres (4 μm in diameter) were produced by dispersion copolymerization of MMA and HEMA in an ethanol‐water medium. Congo Red was attached to the poly(MMA‐HEMA) microspheres, covalently. These Congo Red‐derivatized microspheres were characterized by optical microscopy, Fourier transform infrared spectroscopy, and elemental analysis. Then, Zn(II) ions were incorporated by chelating with the immobilized Congo Red molecules. Different amounts of Zn(II) ions [1.2–17.6 mg of Zn(II)/g of polymer] were conjugated on the microspheres by changing the initial concentration of Zn(II) ions and pH. Bovine serum albumin (BSA) adsorption on these microspheres from aqueous solutions containing different amounts of BSA at different pH and ionic strengths was investigated in batch reactors. The nonspecific BSA adsorption on the plain poly(MMA‐HEMA) microspheres was very low (0.7 mg of BSA/g of polymer). Congo Red derivatization significantly increased the BSA adsorption (up to 35.8 mg of BSA/g of polymer). A further increase in the adsorption capacity (up to 61.0 mg of BSA/g of polymer) was observed when Zn(II) ions were incorporated. More than 90% of the adsorbed BSA was desorbed in 1 h in the desorption medium containing 1.0 M NaSCN at pH 8.0. © 1997 John Wiley & Sons, Inc.